Communications

2019

• C-ACTI - Communications avec actes dans un congrès international

  Beneficial effect of a probiotic prophylactic strategy to counteract neuro-inflammation and behavioral impairments induced by Polycyclic Aromatic Hydrocarbon in mice

  26th International Symposium on Polycyclic Aromatic Compounts (ISPAC), 08-12 septembre, Örebro, Suède

  Morel, C., Schroeder, H., Paoli, J., Charalambous, E., Thiebault, C., Guebels, P., Dosen, A., Turner, J.D., Genay, M., Grova, N.

  See the abstract

  There is growing evidence that supplementation with probiotics improves intestinal transit,
  induces systemic protective immune responses and presents beneficial effect on stress
  and anxiety. Concomitantly, exposure to Polycyclic Aromatic Hydrocarbons (PAH),
  especially in juvenile, proves to induce cognitive developmental delay and behavioral
  impairments related to anxiety.
  This study provides a proof of concept on the use of probiotic beneficial effect to
  counteract the neurotoxic effects induced by PAH. It was carried out by using six groups
  of 12 Swiss female mice each . Three groups were daily fed with a mixture of probiotics
  for 8 weeks whereas the others received the vehicule only. After 1 month of probiotic
  supplementation, the 3 groups of each conditions were exposed by oral gavage to a
  mixture of 16-PAHs (3 times per week, 0, 20 and 200 µg/kg, 4 weeks). Neurobehavioural
  status related to exploration, anxiety and immediate learning were studied during the last
  week of PAH exposure. Faeces were collected, -before, -after 4 weeks of probiotic
  supplementation and -at the end of PAH exposure to assess the microbiota balance and
  the probiotic viability along the gastro-intestinal tract. Preliminary data showed that
  probiotics enable a faster growth of mice compared to controls (p<0.05) and reduce the
  loss of weight observed in PAH-treated groups. Enumeration of probiotic strains at several
  time point, pointed out that the probiotics survive along the gastro-intestinal tract, but PAH
  seems to affect their viability as well as this of microbiota at 200 µg/kg of bw. Analysis of
  microbiota by 16S ribosomal RNA gene sequencing should confirm this result. In the lightdark
  apparatus, supplementation with probiotics partially restore the decrease of the level
  of activity observed in mice exposed to PAH 200 µg/kg (p<0.05). Behavioral analyses
  currently under evaluation should enable us to understand how PAH-induced
  neurotoxicity and if probiotics may prevent their detrimental effects.

• C-ACTN - Communications avec actes dans un congrès national

  Effet bénéfique d'une stratégie prophylactique utilisant un probiotique pour contrebalancer la neuro-inflammation et les altérations du comportement induites par les hydrocarbures aromatiques polycycliques chez la souris

  Microbes – 15ème Congrès National de la Société Française de Microbiologie, 30 septembre - 2 octobre, Paris, France

  Morel, C., Schroeder, H., Paoli, J., Charalambous, E., Thiebault, C., Guebels, P., Dosen, A., Turner, J.D., Genay, M., Grova, N.

  See the abstract Consulter le lien

  Introduction and objectives:

  There is growing evidence that supplementation with probiotics improves intestinal transit, induces systemic protective immune responses and presents beneficial effect on stress and anxiety. Concomitantly, exposure to Polycyclic Aromatic Hydrocarbons (PAH), especially in juvenile, proves to induce cognitive developmental delay and behavioral impairments related to anxiety. This study provides a proof of concept on the use of probiotic beneficial effect to counteract the neurotoxic effects induced by PAH.

  Material and methods:

  We studied six groups of 12 Swiss female mice each. Three groups were daily fed with a mixture of probiotics for 8 weeks whereas the others received the vehicule only. After 1 month of probiotic supplementation, the 3 groups of each conditions were exposed by oral gavage to a mixture of 16-PAHs (3 times per week, 0, 20 and 200 µg/kg, 4 weeks). Neurobehavioural status related to exploration, anxiety and immediate learning were studied during the last week of PAH exposure. Faeces were collected, -before, -after 4 weeks of probiotic supplementation and -at the end of PAH exposure to assess the microbiota balance and the probiotic viability along the gastro-intestinal tract.

  Results, discussion, conclusion:

  Preliminary data showed that probiotics enable a faster growth of mice compared to controls (p<0.05) and reduce the loss of weight observed in PAH-treated groups. Enumeration of probiotic strains at several time point, pointed out that the probiotics survive along the gastro-intestinal tract, but PAH seems to affect their viability as well as this of microbiota at 200 µg/kg of bw. Analysis of microbiota by 16S ribosomal RNA gene sequencing should confirm this result. In the light-dark apparatus, supplementation with probiotics partially restore the decrease of the level of activity observed in mice exposed to PAH 200 µg/kg (p<0.05). Behavioral analyses currently under evaluation should enable us to understand how PAH-induced neurotoxicity and if probiotics may prevent their detrimental effects.

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